A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

Produk Detail:

  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Pages : 420 pages
  • ISBN : 0128226439
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKA Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction
  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Release : 24 April 2020
GET THIS BOOKA Laboratory Guide to the Tight Junction

A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches

Claudins

Claudins
  • Author : Kursad Turksen
  • Publisher : Humana Press
  • Release : 02 July 2011
GET THIS BOOKClaudins

Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters

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A Laboratory Guide to the Mammalian Embryo
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  • Publisher : Oxford University Press
  • Release : 08 January 2004
GET THIS BOOKA Laboratory Guide to the Mammalian Embryo

Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

A Practical Guide to Basic Laboratory Andrology

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  • Author : Lars Björndahl,David Mortimer,Christopher L. R. Barratt,Jose Antonio Castilla,Roelof Menkveld,Ulrik Kvist,Juan G. Alvarez,Trine B. Haugen
  • Publisher : Cambridge University Press
  • Release : 01 April 2010
GET THIS BOOKA Practical Guide to Basic Laboratory Andrology

This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen

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  • Publisher : Springer Science & Business Media
  • Release : 02 December 2021
GET THIS BOOKPhysician's Guide to the Laboratory Diagnosis of Metabolic Diseases

Delay and mistakes in the diagnosis of inherited metabolic diseases may have devastating consequences. Reference laboratory data are scattered and clinical descriptions of rare conditions are hard to locate. This book describes 298 disorders, grouped into 35 chapters according to the type of condition. Within each group of disorders, chapters provide tables of pertinent clinical findings as well as reference and pathological values for crucial metabolites. Relevant metabolic pathways and diagnostic flow charts are included. There are four indices to make the

The Paracellular Channel

The Paracellular Channel
  • Author : Jianghui Hou
  • Publisher : Academic Press
  • Release : 08 June 2018
GET THIS BOOKThe Paracellular Channel

The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure

Human Stem Cell Manual

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  • Author : Jeanne F. Loring,Suzanne Peterson
  • Publisher : Academic Press
  • Release : 02 December 2021
GET THIS BOOKHuman Stem Cell Manual

This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and

A Comprehensive Guide to Toxicology in Nonclinical Drug Development

A Comprehensive Guide to Toxicology in Nonclinical Drug Development
  • Author : Ali S. Faqi
  • Publisher : Academic Press
  • Release : 03 November 2016
GET THIS BOOKA Comprehensive Guide to Toxicology in Nonclinical Drug Development

A Comprehensive Guide to Toxicology in Nonclinical Drug Development, Second Edition, is a valuable reference designed to provide a complete understanding of all aspects of nonclinical toxicology in the development of small molecules and biologics. This updated edition has been reorganized and expanded to include important topics such as stem cells in nonclinical toxicology, inhalation and dermal toxicology, pitfalls in drug development, biomarkers in toxicology, and more. Thoroughly updated to reflect the latest scientific advances and with increased coverage of

A Practical Guide to the Histology of the Mouse

A Practical Guide to the Histology of the Mouse
  • Author : Cheryl L. Scudamore
  • Publisher : John Wiley & Sons
  • Release : 10 February 2014
GET THIS BOOKA Practical Guide to the Histology of the Mouse

A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues. This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical

Neuroglia

Neuroglia
  • Author : Sergey Kasparov
  • Publisher : MDPI
  • Release : 17 June 2019
GET THIS BOOKNeuroglia

Neuroglia is now published as part of Brain Sciences with a new section Editor-in-Chief Prof. Sergey Kasparov.

The Laboratory Mouse

The Laboratory Mouse
  • Author : Hans J. Hedrich
  • Publisher : Academic Press
  • Release : 02 December 2021
GET THIS BOOKThe Laboratory Mouse

The Laboratory Mouse, Second Edition is a comprehensive book written by international experts. With inclusions of the newly revised European standards on laboratory animals, this will be the most current, global authority on the care of mice in laboratory research. This well-illustrated edition offers new and updated chapters including immunology, viruses and parasites, behavior, enrichment and care standards of laboratory mice across the life sciences, medical and veterinary fields. Features four-color illustrations with complete instruction on mouse surgery, anatomy, behavior