Mass Spectrometry in Biophysics

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

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  • Author : Igor A. Kaltashov
  • Publisher : John Wiley & Sons
  • Pages : 320 pages
  • ISBN : 0471705160
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKMass Spectrometry in Biophysics

Mass Spectrometry in Biophysics

Mass Spectrometry in Biophysics
  • Author : Igor A. Kaltashov,Stephen J. Eyles
  • Publisher : John Wiley & Sons
  • Release : 06 May 2005
GET THIS BOOKMass Spectrometry in Biophysics

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical

Biophysical Techniques in Drug Discovery

Biophysical Techniques in Drug Discovery
  • Author : Angeles Canales
  • Publisher : Royal Society of Chemistry
  • Release : 14 November 2017
GET THIS BOOKBiophysical Techniques in Drug Discovery

Biophysical techniques are used in many key stages of the drug discovery process including in screening for new receptor ligands, in characterising drug mechanisms, and in validating data from biochemical and cellular assays. This book provides an overview of the biophysical methods applied in drug discovery today, including traditional techniques and newer developments. Perspectives from academia and industry across a spectrum of techniques are brought together in a single volume. Small and biotherapeutic approaches are covered and strengths and limitations

Mass Spectrometry in Structural Biology and Biophysics

Mass Spectrometry in Structural Biology and Biophysics
  • Author : Igor A. Kaltashov,Stephen J. Eyles
  • Publisher : John Wiley & Sons
  • Release : 03 April 2012
GET THIS BOOKMass Spectrometry in Structural Biology and Biophysics

With its detailed and systematic coverage of the current state of biophysical mass spectrometry (MS), here is one of the first systematic presentations of the full experimental array of MS-based techniques used in biophysics, covering both fundamental and practical issues. The book presents a discussion of general biophysical concepts and a brief overview of traditional biophysical techniques before outlining the more advanced concepts of mass spectrometry. The new edition gives an up-to-date and expanded coverage of experimental methodologies and a

Biophysical Mass Spectrometry Techniques for Probing the Higher-Order Structure of Proteins and Complexes

Biophysical Mass Spectrometry Techniques for Probing the Higher-Order Structure of Proteins and Complexes
  • Author : Sterling, Jr. (Harry John)
  • Publisher : Unknown Publisher
  • Release : 24 July 2021
GET THIS BOOKBiophysical Mass Spectrometry Techniques for Probing the Higher-Order Structure of Proteins and Complexes

Electrospray ionization mass spectrometry (ESI-MS) is a powerful analytical platform for answering a wide variety of questions about the identity, quantity, structure, function, dynamics and energetics of biological molecules. Key advantages of ESI-MS include unrivaled specificity, attomole sensitivity, and the capacity for simultaneous analysis of complex mixtures with analyte masses that differ by less than 1 ppm. The low flow rates and sub-micron sized droplets formed with "nano" ESI allows biomolecular ions to be readily formed from purely aqueous or buffered

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals
  • Author : Damian J. Houde,Steven A. Berkowitz
  • Publisher : Newnes
  • Release : 05 September 2014
GET THIS BOOKBiophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals is concerned with the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure this book takes the reader on a journey on how to best achieve this goal using the key relevant and practical methods commonly employed in the biopharmaceutical industry today as well as up and coming promising methods that are now gaining increasing attention. As a general resource

Development and Application of Mass Spectrometry-based Biophysical Approaches

Development and Application of Mass Spectrometry-based Biophysical Approaches
  • Author : Ying Zhang
  • Publisher : Unknown Publisher
  • Release : 24 July 2021
GET THIS BOOKDevelopment and Application of Mass Spectrometry-based Biophysical Approaches

Mass spectrometry (MS)-based biophysical approaches are new "tools" for protein characterization owing to its capability to analyze proteins and protein complexes that range in molecular weight from kDa to MDa. Protein characterization requires more than identifying the primary structure. More importantly, protein high order structures (i.e., secondary, tertiary and quaternary structures) are needed for biological studies. MS has become the major tool in studies of protein primary structure and post translational modifications (PTMs) over the past two decades.

Modern Biophysical Chemistry

Modern Biophysical Chemistry
  • Author : Peter Jomo Walla
  • Publisher : John Wiley & Sons
  • Release : 22 September 2014
GET THIS BOOKModern Biophysical Chemistry

This updated and up-to-date version of the first edition continues with the really interesting stuff to spice up a standard biophysics and biophysical chemistry course. All relevant methods used in current cutting edge research including such recent developments as super-resolution microscopy and next-generation DNA sequencing techniques, as well as industrial applications, are explained. The text has been developed from a graduate course taught by the author for several years, and by presenting a mix of basic theory and real-life examples,

Methods in Modern Biophysics

Methods in Modern Biophysics
  • Author : Bengt Nölting
  • Publisher : Springer Science & Business Media
  • Release : 09 March 2013
GET THIS BOOKMethods in Modern Biophysics

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for

Proteomics for Biological Discovery

Proteomics for Biological Discovery
  • Author : Timothy D. Veenstra,John R. Yates
  • Publisher : John Wiley & Sons
  • Release : 12 June 2006
GET THIS BOOKProteomics for Biological Discovery

Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics,

Introduction to Biophysical Methods for Protein and Nucleic Acid Research

Introduction to Biophysical Methods for Protein and Nucleic Acid Research
  • Author : Jay A. Glasel,Murray P. Deutscher
  • Publisher : Academic Press
  • Release : 20 November 1995
GET THIS BOOKIntroduction to Biophysical Methods for Protein and Nucleic Acid Research

The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment. Key Features * Modern Use of Mass Spectroscopy * NMR Spectroscopy * Molecular

Novel Mass Spectrometry-based Strategies for Biophysical Analysis of Proteins and Protein-ligand Complexes

Novel Mass Spectrometry-based Strategies for Biophysical Analysis of Proteins and Protein-ligand Complexes
  • Author : Liangjie Tang
  • Publisher : ProQuest
  • Release : 24 July 2021
GET THIS BOOKNovel Mass Spectrometry-based Strategies for Biophysical Analysis of Proteins and Protein-ligand Complexes

The third protocol developed here is a SUPREX-like protocol that employs a chemical modification reaction (i.e., oxidation reaction) in place of the amide H/D exchange reaction in SUPREX. This SUPREX-like protocol, termed SPROX (Stability of Proteins from Rates of Oxidation) was developed and tested to detect and quantify protein-ligand binding using cyclophilin A and BCAII as model systems.

Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen Exchange Mass Spectrometry of Proteins
  • Author : David D. Weis
  • Publisher : John Wiley & Sons
  • Release : 21 March 2016
GET THIS BOOKHydrogen Exchange Mass Spectrometry of Proteins

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
  • Author : Keith Wilson,John Walker
  • Publisher : Cambridge University Press
  • Release : 04 March 2010
GET THIS BOOKPrinciples and Techniques of Biochemistry and Molecular Biology

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate