Cellular Imaging Techniques for Neuroscience and Beyond

The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. * The first comprehensive volume on cellular imaging with a focus for its application in neuroscience * Concluding chapter compares the merits of various techniques * Full color throughout, maximizing users comprehension of the results obtainable via various methods * Features outstanding and truly international scholarship, with chapters written by leading experts in neuroscience and cell biology * Discusses cutting edge methods such as STED, PALM/STORM, nonlinear microscopy and more

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  • Author : Floris G. Wouterlood
  • Publisher : Academic Press
  • Pages : 282 pages
  • ISBN : 0123858720
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKCellular Imaging Techniques for Neuroscience and Beyond

Cellular Imaging Techniques for Neuroscience and Beyond

Cellular Imaging Techniques for Neuroscience and Beyond
  • Author : Floris G. Wouterlood
  • Publisher : Academic Press
  • Release : 14 August 2022
GET THIS BOOKCellular Imaging Techniques for Neuroscience and Beyond

The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document

Neurohistology and Imaging Techniques

Neurohistology and Imaging Techniques
  • Author : Radek Pelc,Wolfgang Walz,Ronald Doucette
  • Publisher : Humana
  • Release : 25 June 2020
GET THIS BOOKNeurohistology and Imaging Techniques

This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed

Optical Probes in Biology

Optical Probes in Biology
  • Author : Jin Zhang,Sohum Mehta,Carsten Schultz
  • Publisher : CRC Press
  • Release : 19 April 2016
GET THIS BOOKOptical Probes in Biology

Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, membrane lipids, voltage, metal ions, metabolic signals, RNA, and histone modifications. It

Visualizing Chemistry

Visualizing Chemistry
  • Author : National Research Council,Division on Earth and Life Studies,Board on Chemical Sciences and Technology,Committee on Revealing Chemistry through Advanced Chemical Imaging
  • Publisher : National Academies Press
  • Release : 01 June 2006
GET THIS BOOKVisualizing Chemistry

Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imaging—the ability to visualize molecular structures and chemical composition in time and space as actual events unfold—from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of

A Primer on Criminal Law and Neuroscience

A Primer on Criminal Law and Neuroscience
  • Author : Stephen J. Morse,Adina L. Roskies
  • Publisher : Oxford University Press
  • Release : 26 July 2013
GET THIS BOOKA Primer on Criminal Law and Neuroscience

(temporary: from the Introduction) As a result, the John D. and Catherine T. MacArthur Foundation decided to support a three-year multidisciplinary initiative, The Law and Neuroscience Project, that created teams (termed "research networks") of lawyers, neuroscientists and philosophers to explore the appropriate conceptual relation of neuroscience and law and to engage in empirical investigations that would demonstrate the specific relevance of neuroscience to law. Although there was a substantial range of opinion among Project participants about the potential relevance of

Optogenetics

Optogenetics
  • Author : Hiromu Yawo,Hideki Kandori,Amane Koizumi,Ryoichiro Kageyama
  • Publisher : Springer Nature
  • Release : 14 August 2022
GET THIS BOOKOptogenetics

This book, now in a thoroughly revised second edition, offers a comprehensive review of the rapidly growing field of optogenetics, in which light-sensing proteins are genetically engineered into cells in order to acquire information on cellular physiology in optical form or to enable control of specific network in the brain upon activation by light. Light-sensing proteins of various living organisms are now available to be exogenously expressed in neurons and other target cells both in vivo and in vitro. Cellular

Optogenetics

Optogenetics
  • Author : Sophie Vriz,Takeaki Ozawa
  • Publisher : Royal Society of Chemistry
  • Release : 18 September 2018
GET THIS BOOKOptogenetics

Optogenetic tools have allowed significant advances in the understanding of biological problems, particularly in the neurosciences field. Biological tools as well as optical set-ups have evolved and a wide range of probes and light-controllable modules are now available. The aim of this book is to give a flavour of illumination strategies and imaging with an overview of the different optogenetic tools and their main applications in cell biology. Based on examples covering the different aspects of cell biology, this book

In Vivo Optical Imaging of Brain Function, Second Edition

In Vivo Optical Imaging of Brain Function, Second Edition
  • Author : Ron D. Frostig
  • Publisher : CRC Press
  • Release : 06 May 2009
GET THIS BOOKIn Vivo Optical Imaging of Brain Function, Second Edition

These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving

Discovering the Brain

Discovering the Brain
  • Author : National Academy of Sciences,Institute of Medicine,Sandra Ackerman
  • Publisher : National Academies Press
  • Release : 01 January 1992
GET THIS BOOKDiscovering the Brain

The brain ... There is no other part of the human anatomy that is so intriguing. How does it develop and function and why does it sometimes, tragically, degenerate? The answers are complex. In Discovering the Brain, science writer Sandra Ackerman cuts through the complexity to bring this vital topic to the public. The 1990s were declared the "Decade of the Brain" by former President Bush, and the neuroscience community responded with a host of new investigations and conferences. Discovering the

Network Neuroscience

Network Neuroscience
  • Author : Flavio Fröhlich
  • Publisher : Academic Press
  • Release : 20 September 2016
GET THIS BOOKNetwork Neuroscience

Studying brain networks has become a truly interdisciplinary endeavor, attracting students and seasoned researchers alike from a wide variety of academic backgrounds. What has been lacking is an introductory textbook that brings together the different fields and provides a gentle introduction to the major concepts and findings in the emerging field of network neuroscience. Network Neuroscience is a one-stop-shop that is of equal use to the neurobiologist, who is interested in understanding the quantitative methods employed in network neuroscience, and

From Neurons to Neighborhoods

From Neurons to Neighborhoods
  • Author : Division of Behavioral and Social Sciences and Education,Institute of Medicine,Board on Children, Youth, and Families,Committee on Integrating the Science of Early Childhood Development
  • Publisher : National Academies Press
  • Release : 13 November 2000
GET THIS BOOKFrom Neurons to Neighborhoods

How we raise young children is one of today's most highly personalized and sharply politicized issues, in part because each of us can claim some level of "expertise." The debate has intensified as discoveries about our development-in the womb and in the first months and years-have reached the popular media. How can we use our burgeoning knowledge to assure the well-being of all young children, for their own sake as well as for the sake of our nation? Drawing from

Live Cell Imaging

Live Cell Imaging
  • Author : Robert D. Goldman,David L. Spector
  • Publisher : CSHL Press
  • Release : 14 August 2022
GET THIS BOOKLive Cell Imaging

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and

Neural Tracing Methods

Neural Tracing Methods
  • Author : Benjamin R. Arenkiel
  • Publisher : Humana Press
  • Release : 06 November 2014
GET THIS BOOKNeural Tracing Methods

This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently

Electrochemical Methods for Neuroscience

Electrochemical Methods for Neuroscience
  • Author : Adrian C. Michael,Laura Borland
  • Publisher : CRC Press
  • Release : 13 December 2006
GET THIS BOOKElectrochemical Methods for Neuroscience

Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical techniques. Today, these methods provide neurochemical information that is not accessible by other means. The growing recognition of the versatility of electrochemical techniques indicates a need for a greater understanding of the scientific foundation and use of these powerful tools. Electrochemical Methods for Neuroscience provides an updated summary of the current, albeit evolving,