FRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

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  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier
  • Pages : 560 pages
  • ISBN : 9780080915128
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKFRET and FLIM Techniques

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier
  • Release : 29 July 2011
GET THIS BOOKFRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier Science
  • Release : 05 January 2009
GET THIS BOOKFRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Multiphoton Microscopy and Fluorescence Lifetime Imaging
  • Author : Karsten König
  • Publisher : Walter de Gruyter GmbH & Co KG
  • Release : 22 January 2018
GET THIS BOOKMultiphoton Microscopy and Fluorescence Lifetime Imaging

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence

Methods in Cellular Imaging

Methods in Cellular Imaging
  • Author : Ammasi Periasamy
  • Publisher : Springer
  • Release : 27 May 2013
GET THIS BOOKMethods in Cellular Imaging

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton

Molecular Imaging

Molecular Imaging
  • Author : Ammasi Periasamy,Richard Day
  • Publisher : Elsevier
  • Release : 28 April 2011
GET THIS BOOKMolecular Imaging

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and

Advanced Time-Correlated Single Photon Counting Techniques

Advanced Time-Correlated Single Photon Counting Techniques
  • Author : Wolfgang Becker
  • Publisher : Springer Science & Business Media
  • Release : 20 December 2005
GET THIS BOOKAdvanced Time-Correlated Single Photon Counting Techniques

In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and

Understanding Light Microscopy

Understanding Light Microscopy
  • Author : Jeremy Sanderson
  • Publisher : Wiley
  • Release : 28 May 2019
GET THIS BOOKUnderstanding Light Microscopy

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and

Integrins and Ion Channels

Integrins and Ion Channels
  • Author : Andrea Becchetti,Annarosa Arcangeli
  • Publisher : Springer Science & Business Media
  • Release : 28 December 2010
GET THIS BOOKIntegrins and Ion Channels

Interdisciplinarity is more often invoked than practised. This is hardly surprising, considering the daunting vastness of modern biology. To reach a satisfactory understanding of a complex biological system, a wide spectrum of conceptual and experimental tools must be applied at different levels, from the molecular to the cellular, tissue and organismic. We believe the multifaceted regulatory interplay between integrin receptors and ion channels offers a rich and challenging field for researchers seeking broad biological perspectives. By mediating cell adhesion to

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy
  • Author : Alberto Diaspro
  • Publisher : CRC Press
  • Release : 26 April 2010
GET THIS BOOKNanoscopy and Multidimensional Optical Fluorescence Microscopy

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular

Leptin and Leptin Antagonists

Leptin and Leptin Antagonists
  • Author : Prof. Arieh Gertler
  • Publisher : CRC Press
  • Release : 06 March 2009
GET THIS BOOKLeptin and Leptin Antagonists

The discovery of leptin, the obese (ob) gene product which is not expressed as a functional protein in ob/ob mice, focused the scientific community’s attention on its role as an anorexic hormone involved in the negative regulation of food intake. Almost 14 years after this breakthrough discovery and over 14,000 leptin-related publications later, leptin is now known to participate in a wide range of biological functions that include, in addition to its early envisaged function as an adipostat, glucose metabolism,

Fluorescence Microscopy

Fluorescence Microscopy
  • Author : Ulrich Kubitscheck
  • Publisher : John Wiley & Sons
  • Release : 27 March 2017
GET THIS BOOKFluorescence Microscopy

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier Science
  • Release : 05 January 2009
GET THIS BOOKFRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Molecular Fluorescence

Molecular Fluorescence
  • Author : Bernard Valeur,Mário Nuno Berberan-Santos
  • Publisher : John Wiley & Sons
  • Release : 27 March 2013
GET THIS BOOKMolecular Fluorescence

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Molecular Interactions

Molecular Interactions
  • Author : Aurelia Meghea
  • Publisher : BoD – Books on Demand
  • Release : 29 February 2012
GET THIS BOOKMolecular Interactions

In a classical approach materials science is mainly dealing with interatomic interactions within molecules, without paying much interest on weak intermolecular interactions. However, the variety of structures actually is the result of weak ordering because of noncovalent interactions. Indeed, for self-assembly to be possible in soft materials, it is evident that forces between molecules must be much weaker than covalent bonds between the atoms of a molecule. The weak intermolecular interactions responsible for molecular ordering in soft materials include hydrogen