FRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

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  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier
  • Pages : 560 pages
  • ISBN : 9780080915128
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKFRET and FLIM Techniques

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier
  • Release : 29 July 2011
GET THIS BOOKFRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques
  • Author : Theodorus W. J. Gadella
  • Publisher : Elsevier Science
  • Release : 05 January 2009
GET THIS BOOKFRET and FLIM Techniques

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Molecular Imaging

Molecular Imaging
  • Author : Ammasi Periasamy,Richard Day
  • Publisher : Elsevier
  • Release : 28 April 2011
GET THIS BOOKMolecular Imaging

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and

Methods in Cellular Imaging

Methods in Cellular Imaging
  • Author : Ammasi Periasamy
  • Publisher : Springer
  • Release : 27 May 2013
GET THIS BOOKMethods in Cellular Imaging

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Multiphoton Microscopy and Fluorescence Lifetime Imaging
  • Author : Karsten König
  • Publisher : Walter de Gruyter GmbH & Co KG
  • Release : 22 January 2018
GET THIS BOOKMultiphoton Microscopy and Fluorescence Lifetime Imaging

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence

Advanced Time-Correlated Single Photon Counting Techniques

Advanced Time-Correlated Single Photon Counting Techniques
  • Author : Wolfgang Becker
  • Publisher : Springer Science & Business Media
  • Release : 20 December 2005
GET THIS BOOKAdvanced Time-Correlated Single Photon Counting Techniques

In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and

FLIM Microscopy in Biology and Medicine

FLIM Microscopy in Biology and Medicine
  • Author : Ammasi Periasamy,Robert M. Clegg
  • Publisher : CRC Press
  • Release : 06 July 2009
GET THIS BOOKFLIM Microscopy in Biology and Medicine

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy’s (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular surroundings of biological

Plant Hormones

Plant Hormones
  • Author : Jürgen Kleine-Vehn,Michael Sauer
  • Publisher : Humana Press
  • Release : 13 September 2016
GET THIS BOOKPlant Hormones

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant

Integrins and Ion Channels

Integrins and Ion Channels
  • Author : Andrea Becchetti,Annarosa Arcangeli
  • Publisher : Unknown Publisher
  • Release : 27 February 2021
GET THIS BOOKIntegrins and Ion Channels

Interdisciplinarity is more often invoked than practised. This is hardly surprising, considering the daunting vastness of modern biology. To reach a satisfactory understanding of a complex biological system, a wide spectrum of conceptual and experimental tools must be applied at different levels, from the molecular to the cellular, tissue and organismic. We believe the multifaceted regulatory interplay between integrin receptors and ion channels offers a rich and challenging field for researchers seeking broad biological perspectives. By mediating cell adhesion to

Time-correlated single photon counting

Time-correlated single photon counting
  • Author : Desmond O'Connor
  • Publisher : Academic Press
  • Release : 02 December 2012
GET THIS BOOKTime-correlated single photon counting

Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight

Optical Imaging Techniques in Cell Biology

Optical Imaging Techniques in Cell Biology
  • Author : Guy Cox
  • Publisher : CRC Press
  • Release : 06 November 2006
GET THIS BOOKOptical Imaging Techniques in Cell Biology

Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E

Fluorescence Lifetime Spectroscopy and Imaging

Fluorescence Lifetime Spectroscopy and Imaging
  • Author : Laura Marcu,Paul M. W. French,Daniel S. Elson
  • Publisher : CRC Press
  • Release : 17 July 2014
GET THIS BOOKFluorescence Lifetime Spectroscopy and Imaging

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Principles of Fluorescence Spectroscopy

Principles of Fluorescence Spectroscopy
  • Author : Joseph R. Lakowicz
  • Publisher : Springer Science & Business Media
  • Release : 17 April 2013
GET THIS BOOKPrinciples of Fluorescence Spectroscopy

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the