Molecular Biology Techniques

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

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  • Author : Sue Carson
  • Publisher : Academic Press
  • Pages : 200 pages
  • ISBN : 0123855446
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKMolecular Biology Techniques

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
  • Publisher : Academic Press
  • Release : 16 January 2021
GET THIS BOOKMolecular Biology Techniques

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Walt Ream,Katharine G. Field
  • Publisher : Academic Press
  • Release : 17 November 1998
GET THIS BOOKMolecular Biology Techniques

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
  • Publisher : Academic Press
  • Release : 05 March 2019
GET THIS BOOKMolecular Biology Techniques

Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into

Morphology Methods

Morphology Methods
  • Author : Ricardo V. Lloyd
  • Publisher : Springer Science & Business Media
  • Release : 15 June 2001
GET THIS BOOKMorphology Methods

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped

Techniques in Molecular Biology

Techniques in Molecular Biology
  • Author : John M. Walker,Wim Gaastra
  • Publisher : Springer Science & Business Media
  • Release : 06 December 2012
GET THIS BOOKTechniques in Molecular Biology

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
  • Author : Keith Wilson,John Walker
  • Publisher : Cambridge University Press
  • Release : 04 March 2010
GET THIS BOOKPrinciples and Techniques of Biochemistry and Molecular Biology

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate

Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology
  • Author : Stefan Surzycki
  • Publisher : Springer Science & Business Media
  • Release : 06 December 2012
GET THIS BOOKBasic Techniques in Molecular Biology

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the

Wood Formation in Trees

Wood Formation in Trees
  • Author : Nigel J Chaffey
  • Publisher : CRC Press
  • Release : 10 January 2002
GET THIS BOOKWood Formation in Trees

Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of

Advances in Molecular Techniques

Advances in Molecular Techniques
  • Author : Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
  • Publisher : CRC Press
  • Release : 11 May 2018
GET THIS BOOKAdvances in Molecular Techniques

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Basic Methods in Molecular Biology

Basic Methods in Molecular Biology
  • Author : Leonard Davis
  • Publisher : Elsevier
  • Release : 02 December 2012
GET THIS BOOKBasic Methods in Molecular Biology

Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three

Pattern Recognition in Computational Molecular Biology

Pattern Recognition in Computational Molecular Biology
  • Author : Mourad Elloumi,Costas Iliopoulos,Jason T. L. Wang,Albert Y. Zomaya
  • Publisher : John Wiley & Sons
  • Release : 24 December 2015
GET THIS BOOKPattern Recognition in Computational Molecular Biology

A comprehensive overview of high-performance pattern recognition techniques and approaches to Computational Molecular Biology This book surveys the developments of techniques and approaches on pattern recognition related to Computational Molecular Biology. Providing a broad coverage of the field, the authors cover fundamental and technical information on these techniques and approaches, as well as discussing their related problems. The text consists of twenty nine chapters, organized into seven parts: Pattern Recognition in Sequences, Pattern Recognition in Secondary Structures, Pattern Recognition in

Methods in Plant Molecular Biology

Methods in Plant Molecular Biology
  • Author : Mary A. Schuler,Raymond E. Zielinski
  • Publisher : Academic Press
  • Release : 02 December 2012
GET THIS BOOKMethods in Plant Molecular Biology

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can

Diagnostic Molecular Biology

Diagnostic Molecular Biology
  • Author : Chang-Hui Shen
  • Publisher : Academic Press
  • Release : 02 April 2019
GET THIS BOOKDiagnostic Molecular Biology

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to aid in the comprehension of this complex subject. Each technique described in this book is explained within its conceptual framework to enhance understanding. The targeted approach covers the principles of molecular biology including the basic knowledge of nucleic acids, proteins, and genomes as well as the basic techniques and instrumentations that are often used in the field of molecular biology with detailed procedures and explanations.