RNA Methodologies

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs

Produk Detail:

  • Author : Robert E. Farrell, Jr.
  • Publisher : Academic Press
  • Pages : 744 pages
  • ISBN : 9780080884950
  • Rating : 4/5 from 21 reviews
CLICK HERE TO GET THIS BOOKRNA Methodologies

RNA Methodologies

RNA Methodologies
  • Author : Robert E. Farrell, Jr.
  • Publisher : Academic Press
  • Release : 31 August 2009
GET THIS BOOKRNA Methodologies

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize

RNA Methodologies

RNA Methodologies
  • Author : Robert E. Farrell
  • Publisher : Academic Press
  • Release : 22 May 2022
GET THIS BOOKRNA Methodologies

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more

RNA Methodologies

RNA Methodologies
  • Author : Gerard Meurant
  • Publisher : Academic Press
  • Release : 02 December 2012
GET THIS BOOKRNA Methodologies

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA

RNA Methodologies

RNA Methodologies
  • Author : Robert E. Farrell, Jr.
  • Publisher : Elsevier
  • Release : 22 July 2010
GET THIS BOOKRNA Methodologies

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more

Recombinant DNA Principles and Methodologies

Recombinant DNA Principles and Methodologies
  • Author : James Greene
  • Publisher : CRC Press
  • Release : 25 June 1998
GET THIS BOOKRecombinant DNA Principles and Methodologies

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel

Epigenetic Regulation and Epigenomics

Epigenetic Regulation and Epigenomics
  • Author : Robert A. Meyers
  • Publisher : John Wiley & Sons
  • Release : 02 October 2012
GET THIS BOOKEpigenetic Regulation and Epigenomics

Epigenetics is a term in biology referring to heritable traits that do not involve changes in the underlying DNA sequence of the organism. Epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance. The "epigenome" is a parallel to the word "genome," and refers to the overall epigenetic state of a cell. Cancer and stem cell research have gradually focused attention on these genome modifications. The molecular basis of epigenetics involves modifications to DNA

RNA Methodologies

RNA Methodologies
  • Author : Robert E. Farrell, Jr.
  • Publisher : Academic Press
  • Release : 11 August 2017
GET THIS BOOKRNA Methodologies

RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from

RNA Methodologies

RNA Methodologies
  • Author : Robert E. Farrell
  • Publisher : Academic Press
  • Release : 22 May 2022
GET THIS BOOKRNA Methodologies

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize

Malaria Methods and Protocols

Malaria Methods and Protocols
  • Author : Denise L. Doolan
  • Publisher : Springer Science & Business Media
  • Release : 22 May 2022
GET THIS BOOKMalaria Methods and Protocols

Despite considerable scientific and medical effort over the past decades, malaria remains the most important human parasitic disease. It is responsible for up to 3 million deaths and another 300-500 million new cases each year, and is becoming resistant to the current chemoprophylactic and chemotherapeutic agents. In Malaria Methods and Protocols, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing,

Neuroanatomical Tract-Tracing

Neuroanatomical Tract-Tracing
  • Author : Laszlo Zaborszky,Floris G. Wouterlood,José Luis Lanciego
  • Publisher : Springer Science & Business Media
  • Release : 22 November 2006
GET THIS BOOKNeuroanatomical Tract-Tracing

The first two editions of this title had a tremendous impact in neuroscience. Between the Second edition in 1989 and today, there has been an explosion of information in the field, including advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from

Handbook of Nucleic Acid Purification

Handbook of Nucleic Acid Purification
  • Author : Dongyou Liu
  • Publisher : CRC Press
  • Release : 14 January 2009
GET THIS BOOKHandbook of Nucleic Acid Purification

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing

Essential Cytometry Methods

Essential Cytometry Methods
  • Author : Zbigniew Darzynkiewicz,J. Paul Robinson,Mario Roederer
  • Publisher : Academic Press
  • Release : 06 October 2009
GET THIS BOOKEssential Cytometry Methods

Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be

Nuclease Methods and Protocols

Nuclease Methods and Protocols
  • Author : Catherine H. Schein
  • Publisher : Springer Science & Business Media
  • Release : 22 May 2022
GET THIS BOOKNuclease Methods and Protocols

Nucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to

Quantitation of mRNA by Polymerase Chain Reaction

Quantitation of mRNA by Polymerase Chain Reaction
  • Author : Thomas Köhler,Dirk Laßner,Anne-Katrin Rost,Barbara Thamm,Barbara Pustowoit,Harald Remke
  • Publisher : Springer Science & Business Media
  • Release : 06 December 2012
GET THIS BOOKQuantitation of mRNA by Polymerase Chain Reaction

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA